To explore the differences of mixed-pixel in spectral mixing mechanism at micro-and macro -scale, the micro- simulation of snow-bare soil mixed pixel was taken as the object of study in an artificial test environment. Reflectance spectra of mixed pixel and snow, bare soil endmember with different area ratio were collected by full-band spectrometer with fixed probe distance. Qualitative and quantitative analysis of original reflectance spectra was done, and reflectance spectra form 350 to 2 500 nm and normalized reflectance spectral data of 350 to 1 815 nm excluding noise were normalized. At the same time, we collected EOS/MODIS and Environment and Disaster Monitoring Satellites data of the same period over the same area and analyzed the correlation of channels in visible, near-infrared and shortwave infrared wavelength range at different resolution scales and the relationship between spectrum of mixed snow-soil and endmember pixel in MODIS image was analyzed. The results showed that, (1) At the micro scale, non-linear relationship existed between mixed pixel and endmember within the scope of the full-wave and linear relationship existed in sub-band wavelength range; (2) At the macro scale, linear relationship existed between mixed pixel and endmember. (3) In statistics of spectral values, the correlation between snow-soil mixture and endmember is positive for snow-soil mixture and snow endmember, and is negative for snow-soil mixture and soil endmember.