Ultraviolet-visible (UV-Vis) and three-dimensional excitation emission matrix fluorescence (3D-EEM) spectroscopies, combined with parallel factor analysis were conducted to investigate the structure and origin of dissolved organic matters (DOM) from soils around Dianchi lake, Kunming. The results showed that the characteristics of all samples’ UV-Visible spectra were alike. The absorption coefficient of DOM samples decreased gradually with the wavelength increasing. There was an obvious shoulder absorption peak in the 250～280 nm band. Additionally, in Chaihe reservoir and the east of Dianchi lake, the aromaticity (A250/A365), molecular weight (SR), humification degree (SUVA254) and hydrophobicity (SUVA260) of soil’s DOM were all much higher than those at other three sampling sites. The 3D-EEM spectrum revealed that all DOM samples around Dianchi lake had four special peaks, which were UV fulvic-like fluorescence, visible fulvic-like fluorescence and two humic-like fluorescences. Fluorescence index (FI) and Autochthonous index (BIX) suggested that soil’s DOM were main from the external import and had low protein-like components and bioavailability. Four fluorescence components were identified with parallel factor analysis and showed significant correlation (p＜0.01), which were illustrated that the four fluorescence components had a similar source. In addition, the contribution rates of fulvic-like fluorescence component was the highest, which suggested that the content of fulvic acid material was relatively higher in soil’s DOM.