The full frame transfer sensor with large array is used in the large array filter-type multi-spectral imaging system, and a mechanical shutter is employed to avoid the images blurred. But the mechanical shutter is unstable, reducing the mosaic images’ uniformity and producing RGB images’ color bar with bias.The paper proposed a method of relative radiance calibration based on objects’ radiance coherence.The correction factors were obtained by the different images with same objects, and used to correct the images based on the first frame. The mosaic images’ first difference was introduced to appraise the images uniformity before and after correction. The field data were corrected by this method, which indicated that the method could correct the non-uniformity of mosaic images and the color bar with bias. The method is unsensitive to the accuracy of mosaicking, and can be used widely.