Spectral data or chrominance data acquired with color measurement device is able to objectively characterize the true color information of the object. As to the measuring target that without direct contact with the measuring instrument, it needs to adopt the non-contact color measurement method to obtain the color information.The spectral irradiance theoretical analysis shows that the changes of the color illumination distance, measuring distance and exposure time have greater impact on the measurement results. However, there is no in-depth study on the influence of the changes of measuring parameters for the measured results. In order to get the most accurate measurement results in the process of non-contact color measurement, parameter optimization study based on orthogonal experiment was proposed. Experiment with different combinations of parameters was conducted to obtain the color information of ColorChecker via PR705 spectroradiometer with range analysis and variance analysis method.Experimental results show that the minimum color difference of measured value and the standard value is 0.878 8ΔE, and the maximum color difference is 1.543 1ΔE. Data results show that the proposed method can effectively select the best combination of parameters, and to analyze the impact of various parameters on the result.