Underwater transportation of bubbles and gases has essential applications in manipulating and using gas, but achieving this function at the microscopic level remains a significant challenge. Here, we report a strategy to self-transport gas in water along a laser-induced open superhydrophobic microchannel with a width less than 100 μm. The femtosecond laser can directly write superhydrophobic and underwater superaerophilic microgrooves on the polytetrafluoroethylene (PTFE) surfaces. In water, the single laser-induced microgroove and water medium generate a hollow microchannel. When the microchannel connects two superhydrophobic regions in water, the gas spontaneously travels from the small region to the large area along this hollow microchannel. Gas self-transportation can be extended to laser-drilled microholes through a thin PTFE sheet, which can even achieve anti-buoyancy unidirectional penetration. The gas can overcome the bubble’s buoyance and spontaneously travel downward. The Laplace pressure difference drives the processes of spontaneous gas transportation and unidirectional bubble passage. We believe the property of gas self-transportation in the femtosecond laser-structured open superhydrophobic and underwater superaerophilic microgrooves/microholes has significant potential applications related to manipulating underwater gas.