Analysis of laser Raman spectroscopy has turned into the efficient research method of substance structure and composition analysis. In the present paper, the principle of laser Raman spectroscopy and its characteristics of analysis method are introduced. Applicability of laser Raman spectroscopy to hydrogen isotope analysis is analyzed. The analytical applications of laser Raman spectroscopy method in radiation reaction of tritium, hydrogen isotope examination and quantitative analysis are presented from reported literature.