To satisfy the requirement of field measurement of optical axis parallelism for the ground-based astronomical observation system, a high-precision multi-optical axis parallel calibration system was put forward. For astronomical observation system which contains the imaging spectrometer, a star was used as a point source, and the star image and its spectrum was synchronous acquisition by using the astronomy tracking system. It was not easy to determine the center of field of view in spectral dimension of the imaging spectrometer, and the scheme takes advantage of control system of the equatorial and a small field of view of the imaging spectrometer, so the center of field of view in spectral dimension was calculated by fitting energy of scanning the target star with respect to the scanning position, then the bias of optical axis parallelism between the instrument was calculated by Gaussian fitting. Experimental results show that the standard uncertainty of this measurement can reach 1.52″, and the optical measurement system structure is simple, so it meets the need of field optical axis parallel measurement with high precision and high speed between the imaging spectrometer and other imaging instrument.