One of the important ways to improve the experimental teaching level and the talent training quality is dismantling, refining and adding the international frontier theories and technologies to the experimental teaching in time. The comprehensive experiment of induction and detection of autophagic flux is designed, which includes cell passage culture, cell transfection and induction and observation of autophagy. A systematic, comprehensive and exploratory modular experimental teaching content is formed. Through double fluorescent labeled autophagosome marker protein expressed in cells and starvation inducing, autophagosomes and autophagic flux are observed by the fluorescence microscope. The practice results in undergraduate experimental teaching indicate that it is helpful to cultivate students’ scientific research thinking and the innovative spirit, and improve their ability to analyze and solve problems.