Attitude disturbance caused by the random impact of airstream is the main disturbance source of the aircraft opto-electronic system. At the effect of the axis friction and mass imbalance of the system, the line-of-sight(LOS) stabilization is affected seriously due to the disturbance. In order to effectively suppress the influence of disturbance moment, a two-axis opto-electronic system dynamic model with outer pitch and inner azimuth was established, and the disturbance transfer relationship which contained the disturbance factors and movement coupling was obtained. A disturbance torque estimated method with extended Kalman filter(EKF) based on the dynamics model was proposed, and the feed-forward control loop of disturbance torque was established, consequently the disturbance torque was compensated at the real time, the system LOS stabilization was improved substantially. A semi-physical simulation experiment using flght simulation test table was carried out. The experiment result shows that the RMS result of LOS stabilization accuacy is 0.026 4 mrad of pitch and 0.029 0 mrad of azimuth frame with feed-forward method, which improve 64.1% and 69.6% compared to disturbance observer (DOB) method.