Based on the data of the scientific experiment in Urad Front Banner for UAV Remote Sensing Load Calibration Field project, with the help of 6 hyperspectral radiometric targets with good Lambertian property, the wide-view multispectral camera in UAV was calibrated adopting reflectance-based method. The result reveals that for green, red and infrared channel, whose images were successfully captured, the linear correlation coefficients between the DN and radiance are all larger than 99%. In final analysis, the comprehensive error is no more than 6%. The calibration results demonstrate that the hyperspectral targets equipped by the calibration field are well suitable for air-borne multispectral load in-flight calibration. The calibration result is reliable and could be used in the retrieval of geophysical parameters.