The effect of sheep manure amendment on soil manganese fractions was conducted in a 11 year experiment at inner Mongolia grassland, using sequential extraction procedure in modified Community Bureau of Reference, and determined by atomic absorption spectrophotometer. Five treatments with dry sheep manure addition rate 0, 50, 250, 750, and 1 500 g·m-2·yr-1, respectively, were carried out in this experiment. Results showed that the recovery rate for total Mn was 91.4%～105.9%, as the percentage recovered from the summation of the improved BCR results with aqua regia extractable contents, and it was 97.2%～102.9% from Certified Soil Reference Materials. Plant available exchangeable Mn could be enhanced by 47.89%, but reducible and total Mn contents decreased significantly under heavy application of manure at depth of 0～5 cm. The effect of manure amendment on Mn fractions was greater in 0～5 cm than in 5～10 cm soil layer. The results are benefit to micronutrient fractions determination and nutrient management in grassland soils.