To investigate the effects of zinc concentration on the growth of Lactobacillus acidophilus and other elements in the bacteria by inductively coupled plasma mass spectrometry (ICP-MS). The potential mechanism of growth and elemental absorption of Lactobacillus acidophilusafterzinc interventionwas investigatedon the re-equilibrium of inorganic elements in the cells. Lactobacillus acidophilus was used as the experimental bacterial strain, and elemental zinc was used as the experimental element to observe the change of growth rate of Lactobacillus acidophilus caused by the change of zinc concentration in the culture solution. A total of 7 zinc concentration groups were set up, and each group of 6 parallel samples had a zinc concentration ranging from 0 to 1 200 ng·mL-1. The concentration interval of each group was 200 ng·mL-1, and the 0 ng·mL-1 group was the control group. Determination of 43 elements such as zinc (Zn), copper (Cu), iron (Fe) and manganese (Mn) in culture medium and Lactobacillus acidophilus by inductively coupled plasma mass spectrometry (ICP-MS); The photometer measures the OD value of Lactobacillus acidophilus and monitors its growth rate. Statistical analysis was performed on the obtained experimental data using SPSS 20.0 (Statistical Package for the Social Science 20.0) statistical software. The change of Zn concentration in the culture solution caused the stimulation effect of Lactobacillus acidophilus growth. The stimulating effect was most obvious when the concentration of Zn was 600 ng·mL-1. At the same time, the concentration of inorganic elements in the bacteria changed. The bacteria included Se and Fe in the bacteria. The content of some beneficial elements in the interior increases with the increase of bacterial growth rate, and the content of harmful elements such as Cd decreases. Correlation analysis showed that the content of 11 elements in Se, U, Fe, Al, Ti, Sb, Ba, Mn, Co, Zn, Sr (arranged from high to low according to the overall correlation coefficient of 2～10 hours) There is a good correlation between OD values, in which U, Al, Ti and Sb are negatively correlated, and the other 7 elements are positively correlated; Se, Sr, Al, Mn, Fe, Co, Cd, etc. in Lactobacillus acidophilus The content of 14 elements was significantly correlated with the content of zinc in the cells (p<0.05). The change of zinc concentration in the culture solution will stimulate or inhibit the growth rate of Lactobacillus acidophilus. At the same time, it can also up-regulate some beneficial elements and down-regulate the content of some harmful elements; the rebalancing of beneficial and harmful elements in the bacteria Perhaps the potential mechanism by which zinc in the culture fluid changes the growth rate of Lactobacillus acidophilus.