For identification of the binder used in Chinese painted cultural relics, the micro-Raman spectroscopy was used to test the pig skin glue, pig bone glue, egg yolk, egg white and peach glue which were commonly used as binder in the relics. The difference between proteinaceous binder and peach glue are distinctly. According to the analysis of their Raman spectrum, the vibrations located at 1 463 and 1 088 cm-1are the features of peach glue. The four kinds of proteinaceous binder also have respective features of their own, although there are many common features in their Raman spectrum. The vibration of 1 737 cm-1 is the feature of pig skin glue, and tie pig bone glue has the feature of vibration located in 962 cm-1. The vibrations of 1 535～1 555 and 759 cm-1 are the features of egg, including egg yolk and egg white. And the egg yolk has another vibration located at 1 749 cm-1 which is different from egg white. It was concluded that the binder can be identified based on the features of Raman spectrum.