Dy3+ doped fluoride borate glass phosphors are prepared with high temperature melting method. In addition, the fluorescence spectra of glass phosphors are determined under the 453nm blue laser using an integrating sphere test system, and the related absolute fluorescence parameters of glass phosphors are obtained. Experimental and calculation results reveal that net emission spectrum power, net emission photon number and the quantum yield are identified to be 286.91 μW, 17.17×1014 cps and 25.86% in 1.0 Wt% Dy2O3 doped fluoride borate glass phosphor under the excitation of blue laser with 15.81 mW, respectively. In order to improve the quality of white light combination, 1.5 Wt% Dy2O3 doped glass phosphor is synthesized to increase the utilization of pump laser and reduce residual blue laser. The white-light emission is obtained under the excitation of blue laser with high powers, and color coordinates of white luminescence are derived to (0.316, 0.287) and (0.303, 0.268) under the excitation of pumping laser with 56.0 and 252.7 mW exciting powers, respectively. The efficient white luminescence under the laser excitation indicates that Dy3+ doped fluoride borate glass phosphors have good application prospect in laser illumination area.