Five kinds of Salen Mn(Ⅱ) and two kinds of Eu(Ⅲ) complexes were synthesized. Under the system of NBT/L-methionine, which creats and detects O·-2, the complexes of antioxidant activity were studied, and the conclusion revealed that all Mn(Ⅱ) complexes have obvious antioxidant activity. The fluorescence study indicated that all the Salen metal complexes could bind with DNA, which shows their potential anticancer activity.