Wangting Zhou1,2,3,4, Hui Xie1,2,3, Kezhou Li1,2,3, Zhiyuan Sun1,2,3..., Jiangshan He1,2,3, Zhen Yuan5, Xunbin Wei6,7,8,* and Xueli Chen1,2,3,4,*|Show fewer author(s)
Author Affiliations
1Xidian University, School of Life Science and Technology, Center for Biomedical-photonics and Molecular Imaging, Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xi’an, China2Xidian University, School of Life Science and Technology, International Joint Research Center for Advanced Medical Imaging and Intelligent Diagnosis and Treatment, Xi’an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, Xi’an, China3Xidian University, Ministry of Education, Engineering Research Center of Molecular and Neuro Imaging, Xi’an, China4Xidian University, Guangzhou Institute of Technology, Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou, China5University of Macau, Faculty of Health Sciences, Macau, China6Peking University Cancer Hospital & Institute, Ministry of Education, Laboratory of Carcinogenesis and Translational Research, Beijing, China7Peking University, Biomedical Engineering Department, Beijing, China8Peking University Health Science Center, Institute of Medical Technology, Beijing, Chinashow less
DOI: 10.1117/1.APN.3.4.046001
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Wangting Zhou, Hui Xie, Kezhou Li, Zhiyuan Sun, Jiangshan He, Zhen Yuan, Xunbin Wei, Xueli Chen, "Flexible depth-of-focus, depth-invariant resolution photoacoustic microscopy with Airy beam," Adv. Photon. Nexus 3, 046001 (2024)
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Abstract
Optical-resolution photoacoustic microscopy (OR-PAM) has rapidly developed and is capable of characterizing optical absorption properties of biological tissue with high contrast and high resolution (micrometer-scale lateral resolution). However, the conventional excitation source of rapidly diverging Gaussian beam imposes limitations on the depth of focus (DOF) in OR-PAM, which in turn affects the depth-resolving ability and detection sensitivity. Here, we proposed a flexible DOF, depth-invariant resolution photoacoustic microscopy (FDIR-PAM) with nondiffraction of Airy beams. The spatial light modulator was incorporated into the optical pathway of the excitation source with matched switching phase patterns, achieving the flexibly adjustable modulation parameters of the Airy beam. We conducted experiments on phantoms and intravital tissue to validate the effectiveness of the proposed approach for high sensitivity and high-resolution characterization of variable topology of tissue, offering a promising DOF of 926 μm with an invariant lateral resolution of 3.2 μm, which is more than 17-fold larger compared to the Gaussian beam. In addition, FDIR-PAM successfully revealed clear individual zebrafish larvae and the pigment pattern of adult zebrafishes, as well as fine morphology of cerebral vasculature in a large depth range with high resolution, which has reached an evident resolving capability improvement of 62% mean value compared with the Gaussian beam.
