A large-scale multi-projection light-field 3D display system based on multi-view sampling calibration was proposed. In this system 360 projectors were arranged in a circle under an anisotropic cylindrical diffuser with 3 m diagmeter and 1.8 m height and projecting images onto the diffuser. Light field of 3D scenes was accurately reconstructed inside the screen and can be observed by multiple viewers from different angles and positions simultaneously around 360°, as well as providing smooth motion parallax. Rendering frame rate of dynamic scenes can achieve 30 frame/s and above, offering fluent visual experience. To realize this system, a wide-field projection lens with cylindrical lenses was designed to enlarge image of the projectors. Then an automatic light-field calibration method based on multi-view sampling was proposed to eliminate distortion, which was induced by non-linear distortion of the projection lens and system assembly error, from the projection images. After calibration, light field projected by 360 projectors can be fuszed together seamlessly.