In order to clarify the relationship between aromatic hydrocarbon components and micro-fluorescence spectra in oil inclusions, based on the fluorescence property of oil, the fluorescence spectra of oil inclusions in Gaotaizi reservoir in Qijia area of Songliao Basin were quantitatively described by non-destructive analysis of single inclusion components. Firstly, the fluorescence color types of oil inclusions in reservoirs were obtained, and then the fluorescence spectra of single oil inclusions were obtained. The main peak wavelength characteristic values of standard aromatics under 365 nm monochrome light excitation were compared. Finally, the hydrocarbon charging episodes and the types of aromatics in different episodes of oil inclusions were classified. The results show that there are three kinds of fluorescent oil inclusions: yellow, yellow-green and blue-green, which represent hydrocarbons charging with different maturity. The main aromatic components in oil inclusions are benzotetraphenyl, decacyclene and benzophenanthrene, followed by bilianthracene and a small amount of pentacene and red fluorene. Among them, the aromatic components in the first screen oil inclusion are pentacene, benzotetraphenyl, red fluorene and decacyclene; the aromatic components in the second screen oil inclusion are mainly benzotetrabenzene, decacyclene, benzophenanthrene and a small amount of red fluorene; and the third screen oil inclusion is benzophenanthrene. The main aromatic hydrocarbon components are benzotetraphenyl, decacyclene and benzophenanthrene, followed by bilianthracene. According to the types of aromatic hydrocarbon components, there are more high molecular weight aromatic hydrocarbons in Episode 1 than in Episode 2 and episode 3, showing lower maturity, while there are more types of small molecular aromatic hydrocarbons in Episode 2 and episode 3, showing medium maturity. Reservoir oil inclusions generally show fewer aromatics of small molecular weight and more aromatics of large molecular weight, which indicates that crude oil has undergone biodegradation and water washing before being captured by inclusions, and has undergone overheating and metamorphism after capturing. Crude oil in reservoir inclusions mainly consists of low-to medium-maturity crude oil. Finally, the relationship between the fluorescence spectrum characteristics of oil inclusions and the aromatics components is drawn up, which provides a basis for the study of the types and maturity of aromatics components in crude oil.