Abalone and sea cucumbers are useful,which is related with trace elements in them.In the present paper,using high-pressure sealed nitrifying pots for digestion of samples,Orthogonal test was applied to the determination of 15 kinds of trace elements in abalone and sea cucumber such as Fe,Ca,Mg,Zn,Sr,Se,Hg,Cd etc by ICP-AES.The orthogonal table of L16 (44) and experiment programs were designed.More information could be obtained with a small number of experimental times by combining chemistry with mathematical statistics,and the best experimental operation conditions could be chosen by square-error analysis.The method was simple,rapid,highly sensitive and accurate,but also features multi-element determination at the same time,with little environment pollution.The recovery rate of the method is in the range of 91.0%-110.0%,and relative standard deviations is less than 3.55%.The experimental results showed that both abalone and sea cucumber are of nutritional value of seafood,and are rich in Fe,Ca,Mg,Zn,Sr and other kinds of trace elements helpful to the human body.At the same time,because of severe environment pollution,seawater pollution should not be neglected.When seawater was polluted,hazardous substances were deposited in abalone or sea cucumber body with high levels of harmful heavy metals.Therefore,it should be a reminder that people must be careful to eat them.