The regulation of circadian rhythm by light is a non-imaging visual function.Non-imaging visual effect cells, mainly intrinsically photosensitive retinal ganglion cells, significantly impact the body’s biological rhythm, metabolism, cognition, etc., primarilymediated by blue light. This study aimed to explore the effects of night light exposure on liver circadian rhythm and inflammation. The gerbils were exposed to white and blue light for 1 or 3 hours every night for 10 weeks. The real-time reverse transcription-polymerase chain reaction was used to detect the expression rhythm of liver clock genes; transcriptomics was used to analyze the expression level of all liver genes; hematoxylin-eosin staining was used to detect liver tissue morphology, and immunofluorescence staining was used to detect the level of inflammatory factors in liver tissue. The results showed that light exposure at night significantly disrupted the liver’s expression rhythm of Arntl, Clock, Cry1, Nr1d1, Per2, and Rora. Light exposure at night had the most significant effect on liver immune system gene expression. Light exposure at night induced hepatocyte edema and up-regulated the expression of pro-inflammatory factors interleukin 17A and granulocyte-macrophage colony-stimulating factor. In short, exposure to light at night disrupted liver circadian rhythms and triggered an inflammatory response in the liver. This study highlights the critical role of non-imaging visual pathways in hepatic metabolic homeostasis.