A novel rice-like copper oxide (CuO) was synthesized without using soft template and alkali by hydrothermal and in-situ decomposition methods. This rice-like CuO material was made into the chemically modified electrode (CME) with Nafion solution for non-enzymatic glucose sensing. Structure and morphology of the prepared material and electrode were characterized by X-ray diffraction and scanning electron microscopy. Electrochemical performances of the obtained electrodes were investigated by linear sweep voltammetry, cyclic voltammetry, amperometric response, and electrochemical impedance spectroscopy. Results show that morphology of the obtained CuO particle is similar to rice grain. And its length and diameter are between 0.5-1.0 μm and 250-320 nm, respectively. The CME with 0.35 mg rice-like CuO (with 0.22 cm² electrode surface area) has an obvious current response for glucose with the linear range from 0.0357 to 2.361 mmol/L, the linear equation I_pa(mA)= -0.00187+0.05239 c(mmol/L) (R²=0.998), the detection limit 0.0647 μmol/L, and the sensitivity 950.36 μA·L/(mmol·cm²). Therefore, the prepared CuO CME shows a promise selectivity and reliability for detecting glucose.