A quantum signature protocol based on quantum one-time pad and quantum key distribution was presented, in which the arbitrage didn’t participate in. In the process of signature, message was transformed to qubit, and the identity information of signer was attached to it. After signed, the signature message was transfered into frame structure by signer, and was transported to receiver. In the process of validation, both of communication sides must validate identity information and preserve this information each other, before the receiver wanted to obtain the information of the frame completely. Therefore the signature protocol attains the goal of non-denial.