Labor intensive, time consuming, high technical requirements in operation and much affected by human factors is the limitation of diagnosing the crop information with conventional method, which could not make diagnosis real-time and rapid. Imaging spectral technique could simultaneously obtain the image and spectral information of crops. It could diagnose the growth and insects information of crop rapidly and non-destructively. In recent years, imaging spectroscopy has been widely used in diagnosis of the information of crop, so it provides technical support for agricultural informatization. In the present study, the principle of imaging spectroscopy was presented. The application progress of imaging spectroscopy technique in crop detection was investigated, including seed component detection, seed variety discrimination, seed disease and insect pest detection, field crop growth monitoring and field crop disease and insects monitoring. Then the paper analyzed difficulty of imaging spectroscopy for crop measurement, and the prospect of this technique was also discussed.