• Journal of Inorganic Materials
  • Vol. 39, Issue 5, 517 (2023)
Heqing CAI1, Lu HAN1,*, Songsong YANG1, Xinyu XUE1..., Kou ZHANG1, Zhicheng SUN1, Ruping LIU1, Kun HU1 and Yan WEI2,*|Show fewer author(s)
Author Affiliations
  • 11. School of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing 102600, China
  • 22. Key Laboratory of Organophosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084, China
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    DOI: 10.15541/jim20230464 Cite this Article
    Heqing CAI, Lu HAN, Songsong YANG, Xinyu XUE, Kou ZHANG, Zhicheng SUN, Ruping LIU, Kun HU, Yan WEI. Fe3O4-DMSA-PEI Magnetic Nanoparticles with Small Particle Size: Preparation and Gene Loading [J]. Journal of Inorganic Materials, 2023, 39(5): 517 Copy Citation Text show less

    Abstract

    Ferroferric oxide (Fe3O4) magnetic nanoparticles are widely used as passive targeting carriers in gene therapy, due to their simple preparation, targeting under external magnetic field and easy surface grafting. This study synthesized oil phase Fe3O4 nanoparticles with controllable particle sizes in the range from 4 to 9 nm by regulating the accumulation growth time in the solvothermal method. Then, meso-2, 3-dimercaptosuccinic (DMSA) was employed to double exchange oleic acid molecules on its surface to provide good water dispersibility. Finally, Fe3O4-DMSA-PEI magnetic nanoparticles were obtained by grafting branched polyethylenimine (PEI) onto Fe3O4-DMSA surface through amidization reaction. The results demonstrate that the Fe3O4-DMSA-PEI magnetic nanoparticles have a surface Zeta potential of (52.50 ± 1.94) mV, remaining a certain degree of superparamagnetism (14.48 emu/g, 1 emu/g=1 A∙m2/kg). When the mass ratio of Fe3O4-DMSA-PEI magnetic nanoparticles to plasmid DNA is 15 : 1, it can completely block DNA and its loading capacity is as high as 6.67%. The Fe3O4-DMSA-PEI magnetic nanoparticles prepared in this study have a certain gene delivery ability and are expected to be used as gene carriers in the field of gene transfection.
    Heqing CAI, Lu HAN, Songsong YANG, Xinyu XUE, Kou ZHANG, Zhicheng SUN, Ruping LIU, Kun HU, Yan WEI. Fe3O4-DMSA-PEI Magnetic Nanoparticles with Small Particle Size: Preparation and Gene Loading [J]. Journal of Inorganic Materials, 2023, 39(5): 517
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