• Chinese Optics Letters
  • Vol. 23, Issue 3, 030501 (2025)
Qingyang Yue1,2,3, Hao Ma1,2,3, Yabo Han1,2,3, Hongyi Huang1,2,3..., Xianlong Liu1,2,3, Yang Yang1,2,3,* and Chengshan Guo1,2,3|Show fewer author(s)
Author Affiliations
  • 1Shandong Provincial Engineering and Technical Center of Light Manipulations & Shandong Provincial Key Laboratory of Optics and Photonic Device, School of Physics and Electronics, Shandong Normal University, Jinan 250014, China
  • 2Collaborative Innovation Center of Light Manipulation and Applications, Shandong Normal University, Jinan 250358, China
  • 3Joint Research Center of Light Manipulation Science and Photonic Integrated Chip of East China Normal University and Shandong Normal University, East China Normal University, Shanghai 200241, China
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    DOI: 10.3788/COL202523.030501 Cite this Article Set citation alerts
    Qingyang Yue, Hao Ma, Yabo Han, Hongyi Huang, Xianlong Liu, Yang Yang, Chengshan Guo, "Microsphere-assisted single-shot polarization holographic microscopy for quantitative birefringence imaging of dynamic samples," Chin. Opt. Lett. 23, 030501 (2025) Copy Citation Text show less
    (a) Schematic of the microsphere-assisted high-resolved quantitative birefringence microscopy setup. (b) The detailed description of the beam propagation paths and arrangement of the optical components in the red box of (a).
    Fig. 1. (a) Schematic of the microsphere-assisted high-resolved quantitative birefringence microscopy setup. (b) The detailed description of the beam propagation paths and arrangement of the optical components in the red box of (a).
    (a) Experimental setup of the system constructed based on Fig. 1(a). (b) Example of the AMPH captured by the experimental setup of (a). (c) Fourier spectrum of the AMPH.
    Fig. 2. (a) Experimental setup of the system constructed based on Fig. 1(a). (b) Example of the AMPH captured by the experimental setup of (a). (c) Fourier spectrum of the AMPH.
    Example of the measured birefringence phase retardation and optic-axis orientation maps of the QWP without and with the MS in our MS-SSQBM system. (a), (b) The recovered amplitude distributions associated with two polarization states of the background and sample. (c), (d) The recovered phase distributions associated with two polarization states of the background and sample. (e), (f) The retrieved birefringence phase retardation and optic-axis orientation distributions of the background and sample images, respectively. Scale bar: 20 µm.
    Fig. 3. Example of the measured birefringence phase retardation and optic-axis orientation maps of the QWP without and with the MS in our MS-SSQBM system. (a), (b) The recovered amplitude distributions associated with two polarization states of the background and sample. (c), (d) The recovered phase distributions associated with two polarization states of the background and sample. (e), (f) The retrieved birefringence phase retardation and optic-axis orientation distributions of the background and sample images, respectively. Scale bar: 20 µm.
    Combination of the measured birefringence information curves of the QWP without and with the insertion of the MS in the setup for rotation angles of 0 rad to π rad.
    Fig. 4. Combination of the measured birefringence information curves of the QWP without and with the insertion of the MS in the setup for rotation angles of 0 rad to π rad.
    Example of holographic birefringence imaging of a birefringence resolution target without and with the insertion of an MS in the setup. (a), (b) The recovered amplitude distributions of two orthogonal polarization states of the tested resolution target without and with an MS, respectively. (c), (d) The corresponding phase distributions. (e), (f) The recovered birefringence phase retardation and optic-axis orientation distributions without and with an MS, respectively. Scale bar: 4 µm.
    Fig. 5. Example of holographic birefringence imaging of a birefringence resolution target without and with the insertion of an MS in the setup. (a), (b) The recovered amplitude distributions of two orthogonal polarization states of the tested resolution target without and with an MS, respectively. (c), (d) The corresponding phase distributions. (e), (f) The recovered birefringence phase retardation and optic-axis orientation distributions without and with an MS, respectively. Scale bar: 4 µm.
    Example of the holographic birefringence imaging of a 500 lp/mm holographic grating without and with the insertion of an MS in the setup. (a), (b) The recovered amplitude distributions of two orthogonal polarization states of the grating without and with an MS, respectively. (c), (d) The corresponding phase distributions. (e), (f) The recovered birefringence phase retardation and optic-axis orientation distributions without and with an MS, respectively. (g) The line profiles indicated by the dashed lines in (a). Scale bar: 10 µm.
    Fig. 6. Example of the holographic birefringence imaging of a 500 lp/mm holographic grating without and with the insertion of an MS in the setup. (a), (b) The recovered amplitude distributions of two orthogonal polarization states of the grating without and with an MS, respectively. (c), (d) The corresponding phase distributions. (e), (f) The recovered birefringence phase retardation and optic-axis orientation distributions without and with an MS, respectively. (g) The line profiles indicated by the dashed lines in (a). Scale bar: 10 µm.
    Qingyang Yue, Hao Ma, Yabo Han, Hongyi Huang, Xianlong Liu, Yang Yang, Chengshan Guo, "Microsphere-assisted single-shot polarization holographic microscopy for quantitative birefringence imaging of dynamic samples," Chin. Opt. Lett. 23, 030501 (2025)
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